In vitro studies, 25C-NBOMe is proven to show cytotoxicity on neuronal cell traces SH-SY5Y, PC12, and SN471, plus the compound was much more potent than methamphetamine at minimizing the visibility with the respective cells; the neurotoxicity with the compound involves activation of MAPK/ERK cascade and inhibition of Akt/PKB signaling pathway.25I-N